Course description:
DNA Replication & Polymerase Chain Reaction (PCR)
DNA and gene cloning
genomic and cDNA libraries
DNA sequencing
Molecular markers: Hybridization-based (RFLP, VNTR) and PCR-based (RAPD, AFLP, STS) techniques
SSR markers development (SSR, M-AFLP, SAMPL)
BLAST & Primer3
Qualitative Mapping
Link to Joinmap Website
Download Joinmap Manual
Download evaluation copy of Joinmap 4.0
Subjects of the practicals:
DNA isolation and quantification
gel electrophoresis
PCR and PCR-RFLP
SSR analysis
paternity test using molecular markers
Examination methods:
mid-term and final test evaluation
Course description:
Link to a FREE BOOK on Marker-Assisted Selection (FAO)
Molecular Markers
Use of molecular markers for genotyping
Molecular phylogenesis and bootstrapping
Genetic mapping of qualitative traits
Link to Joinmap Website
Download Joinmap Manual
Download evaluation copy of Joinmap 4.0
Mapping of QTLs
MAS (marker assisted selection)
Genomic (classical, BACs, YACs) and transcriptomic libraries construction
Methods for isolating differentially expressed genes (Differential display, cDNA-AFLP)
BLAST analysis
In situ hybridization methods and FISH technique
Subjects of the practicals:
AFLP analysis (protocol)
Scoring of AFLP gels
Mapping
Transformation
Western blotting
Examination methods:
weekly paper discussion and final test evaluation
more info
Course description:
Introduzione alla genetica
Il DNA come materiale ereditario
Richiami di meiosi e mitosi dal punto di vista genetico
Le esperienze di Mendel e il Chi quadro
Associazione, mappaggio e mappatura
Cromosomi ed eredità
Estensioni del Mendelismo
Mutazioni
Genetica dei microrganismi (Achilli)
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