Course description:

• Link to a FREE BOOK on Marker-Assisted Selection (FAO)
  
• The big world of Molecular markers and Marker Assisted Breeding. English CFU.
• Molecular Markers
• Use of molecular markers for genotyping
• Molecular phylogenesis and bootstrapping
• Genetic mapping of qualitative traits
• Link to Joinmap Website
  
• Download Joinmap Manual
  
• Download evaluation copy of Joinmap 4.0
  
• Mapping of QTLs
• MAS (marker assisted selection)
• Genomic (classical, BACs, YACs) and transcriptomic libraries construction
• Methods for isolating differentially expressed genes (Differential display, cDNA-AFLP)
• BLAST analysis
• In situ hybridization methods and FISH technique

Subjects of the practicals:

• AFLP analysis (protocol)
• Scoring of AFLP gels
• Mapping
• Transformation
• Western blotting

Examination methods:

• weekly paper discussion and final test evaluation

Course description:

• Restriction enzymes, electrophoresis and restriction maps
• Southern blotting
• Polymerase chain reaction (PCR)
• DNA and gene cloning
• genomic and cDNA libraries
• DNA sequencing
• Molecular markers: Hybridization-based (RFLP, VNTR) and PCR-based (RAPD, AFLP, STS) techniques
• SSR markers development (SSR, M-AFLP, SAMPL)
• BLAST & Primer3
• Qualitative Mapping
• Link to Joinmap Website
  
• Download Joinmap Manual
  
• Download evaluation copy of Joinmap 4.0
  
• Methods for isolating differentially expressed genes (Differential display, cDNA-AFLP)
• BLAST analysis
• use of advanced techniques for DNA analysis in animal breeding: conservation, paternity/genetic distances analysis
• genetic manipulation of animals.

Subjects of the practicals:

• DNA isolation and quantification
• gel electrophoresis
• PCR and PCR-RFLP
• SSR analysis
• paternity test using molecular markers

Examination methods:

• mid-term and final test evaluation

Course description:

• Restriction enzymes, electrophoresis and restriction maps
• Southern blotting
• Polymerase chain reaction (PCR)
• DNA and gene cloning
• genomic and cDNA libraries
• DNA sequencing
• Molecular markers: Hybridization-based (RFLP, VNTR) and PCR-based (RAPD, AFLP, STS) techniques
• SSR markers development (SSR, M-AFLP, SAMPL)
• Methods for isolating differentially expressed genes (Differential display, cDNA-AFLP)
• BLAST analysis
• MAS (marker assisted selection) for conservative selection
• Principles of genetic transformation
• The production of seeds in GMO
• Evaluation of GMO seeds inside traditional seed stocks

Subjects of the practicals:

• DNA isolation, agarose gel and electrophoretic pathway
• Cloning
• Transformation
• PCR analysis
• seed stock analysis for GMO

Examination methods:

• mid-term and final test evaluation